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康复训练联合促红细胞生成素对脑出血大鼠神经 修复及海马胶质原纤维酸性蛋白表达的影响
林涛周俊香杨菁杨卫远林芳芳
()
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目的 康复训练联合促红细胞生成素(EPO)对脑出血大鼠神经修复及海马组织胶质原纤 维酸性蛋白(GFAP)表达的影响。方法 健康雄性SD 大鼠30 只随机分成3 组:模型组、EPO 组和训练组, 每组各10 只大鼠。3 组均建立脑出血模型,EPO 组与训练组在造模成功24 h 后均腹腔注射EPO,训练组 大鼠给予康复训练,模型组注射与EPO 组及训练组所注射EPO等量的生理盐水。观察与检测大鼠神经 修复及海马GFAP表达情况。结果 所有大鼠均造模成功,EPO 组与训练组造模后7 d、14 d 和28 d 的 Tarlov 评分高于模型组(P< 0.05),训练组也显著高于EPO 组(P < 0.05)。造模后28 d,EPO 组与训练组 的脑含水量、血清IL-6 和TNF-α 值、海马组织GFAP蛋白相对表达水平显著低于模型组(P< 0.05),训练 组也低于EPO 组(P < 0.05)。结论 康复训练联合EPO在脑出血大鼠中的应用能抑制海马GFAP表达 与血清炎性因子的释放,减轻脑水肿,从而促进神经功能恢复正常。
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Effects of rehabilitation training combined with erythropoietin on nerve repair and expression ofGFAP in hippocampus of rats with cerebral hemorrhage
Lin Tao, Zhou Junxiang, Yang Jing, Yang Weiyuan, Lin Fangfang
()
Abstract:
Objective To evaluate the effects of rehabilitation combined with erythropoietin( EPO) on nerve repair and expression of glial fibrillary acidic protein( GFAP) in rats with cerebral hemorrhage. Methods A total of 30 healthy male Sprague-Dawley rats were randomly divided into 3 groups- model group and EPO group and training group, with 10 rats in each group. The cerebral hemorrhage model was established in the three groups. After 24 hours of modeling, rats in the EPO group and the training group were given intraperitoneal injection of EPO, the rats in the training group were also given rehabilitation training, and the model group were injected with the same amount of saline as the amount of EPO the EPO group and the training group received. The rats' nerve repair and hippocampal GFAP expression situation were observed and detected. Results All rats were successfully modeled, the Tarlov scores of the EPO group and the training group at 7 d, 14 d and 28 d after modeling were higher than those of the model group( P < 0.05), with the training group significantly higher than the EPO group( P<0.05). At 28 days after modelling, the brain water content, serum IL-6 and TNF-α values, and the relative expression levels of GFAP protein in hippocampus were significantly lower in the EPO group and the training group than in the model group( P<0.05), with the training group lower than the EPO group( P < 0.05). Conclusions The combination of rehabilitation training and erythropoietin in rats with cerebral hemorrhage can inhibit the expression of GFAP in hippocampus and the release of serum inflammatory factors, reduce brain edema, and promote the restoration of normal nerve function.

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